Triple quadruple mass spectrometer with multiple reaction monitoring is considered to be a gold standard in targeted analysis of metabolites. Thermo’s TSQ Altis Triple-Stage Quadrupole Mass Spectrometer is one of the high-end machine in this category. Its segmented quadrupoles and active ion management technology (AIM+) provides higher sensitivity, robustness, reliability and consistency to quantitative analyses. The fast high resolution selected reaction monitoring (H-SRM) technology, polarity switching functions and mixed mode operations makes it a highly recommended platform for quantification of small molecules. It is connected to a Dionex Ultimate 3000 UHPLC system and this LC-MS/MS system is being employed for all the targeted assay development at IOB.

Q Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer with a high capacity transfer tube and electrodynamic ion funnel that maximize ion loading and transmit ions over a broad mass range. This leads to high sensitivity and excellent stability in analysis quality from run to run. The robust quantitative workflows, including parallel reaction monitoring (PRM), high-resolution data-dependent acquisition (HR-DDA) and high-resolution data-independent acquisition (HR-DIA) help in the metabolomics and lipidomics analysis.